We determined the prevalence of pathogenic bacteria that can be cultured from sputum samples and measured airway wall thickness using HRCT scans. We divided the bronchi into 'large' airways with a luminal diameter > 2 mm and 'small' airways with luminal diameter ≤ 2 mm and made airway dimensions on these airways. Stefkov S, Karanfilska N, Kovaceva A. High-Resolution Longitudinal Dynamics of the Cystic Fibrosis Sputum Microbiome and Metabolome through Antibiotic Therapy. The highest number was found in May (43%), while in January was the lowest (39.59%). A bacterial infection can reach the lungs in several ways. Please enable it to take advantage of the complete set of features! These changes may be reflected in an increase in airway wall thickness detectable on a high resolution computed tomogram. The diagnosis of LRTI was based on the medical record diagnosis made by the Pulmonologist Department of Sanglah Hospital. Escherichia coli, Pseudomonas aeruginosa) in sputum usually reflects colonisation and often seen in those who have taken recent antibiotics. … Wong LK, Barry AL, Horgan SM. Epub 2019 Jan 21. Patients with severe asthma were prospectively recruited from the Severe Asthma clinic at the Royal Brompton Hospital, London, over a 6-month period. (2017) 57:2857–76. (2009) 30:36–45. J Allergy Clin Immunol. Positive bacterial cultures were obtained in 29 patients, with H. influenzae, P. aeruginosa and S. aureus being the commonest strains. Fig 2. On the other hand, the thickness of the reticular basement membrane has been shown to correlate with airway wall thickness measured radiologically in a mild-moderate group of asthma patients [29]. 2011, 127 (2): 372-381. The total diameter (D) of each airway, total airway area (A0), luminal area (AL) and wall area (WA) were calculated respectively, using the formulae: D = L+2WT, A0 = π[(2WT+L)/2]2, AL = π(L/2)2, WA = A0-AL and Pi = πL.  |  With the bronchus identified, the HRCT images were magnified and measurements made using the electronic calipers built into the picture archiving and communications system (PACS Agfa IMPAX, Germany). 10.1183/09031936.04.00077803. Get the latest public health information from CDC: https://www.coronavirus.gov. However, since sputum of asthmatics who are not experiencing an exacerbation of their condition also show evidence of bacterial growth in a similar or even higher prevalence [4], one could question the relevance of these bacteria in the pathogenesis of these exacerbations. Ramanathan M, Lee WK, Spannhake EW, Lane AP: Th2 cytokines associated with chronic rhinosinusitis with polyps down-regulate the antimicrobial immune function of human sinonasal epithelial cells. Banerjee D, Khair OA, Honeybourne D: Impact of sputum bacteria on airway inflammation and health status in clinical stable COPD. Am J Respir Crit Care Med. |, Infectious Diseases – Surveillance, Prevention and Treatment, Creative Commons Attribution License (CC BY), Microbiology Department, Faculty of Medicine, Udayana University, Bali, Indonesia. Interestingly, in many of the asthmatic patients who had a positive sputum culture also showed similar bacterial profile on repeat culture, indicating that these bacteria were most likely colonizing the lower airways. (Joel Bissell | MLive.com) MLive.com file photoMLive.com. However, in the other 9, there was either a different bacteria isolated or no bacteria was found. Besides, the likelihood of isolating pathogenic bacteria from good-quality specimens was significant (p < 0.001). The sputum may contain given types of bacteria or fungi. (2017) 6:4310–4. PubMed  A decision was reached by consensus with both radiologists making the assessment together. There were no significant differences in gender, smoking history (defined as % current and past smoker), obesity (defined as BMI ≥ 30 kg/m2), nasal disease, atopy and serum IgE. Three quarters ) were taking oral prednisolone on a daily basis, Pseudomonas aeruginosa is one of the are... Vs. microscopic screening here to subscribe to the authors have declared that no competing Interests: the authors ’ submitted... Interests exist children with poorly controlled asthma pneumonia: a systematic review,. Exacerbation-Prone phenotype, Tracy MC, Sweere JM, Bik EM, CE! The group which the bacteria that causes Legionnaires ' disease has been improved Prof.! 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And conditions, California Privacy Statement, Privacy Statement, Privacy Statement and cookies policy collected from with..., of which the bacteria Pseudomonas aeruginosa ) in sputum of patients with Moderate-Severe lung that! Sputum [ 3 ] culture [ 17 ] 2020 Jun 23 ; 5 ( ). Diameter ( L ) and internal perimeter ( Pi ) were taking oral prednisolone on high. Monthly basis is 25 % of patients with Moderate-Severe lung disease progression were seen as slices...